What is PAS solution?
Summary of the Article
PAS (Periodic Acid Schiff) Stain is used as an immunohistochemical method on biopsies as well as a diagnostic tool for blood specimens. Psoralen-treated platelets do not require irradiation and, therefore, do not have a Rad-Sure™ sticker on the bag. Psoralen-treated platelets are platelets that undergo treatment with a psoralen drug (amotosalen) followed by UV light to prevent DNA or RNA replication. Platelets that have been pathogen-reduced by psoralen treatment and UVA do not need to be irradiated, as this method is considered equivalent to irradiation by the FDA. PAS-positive substances include polysaccharides like glycogen, cellulose, and starch, which can be found in various organisms. PAS staining is commonly used in the histology laboratory to detect glycogen deposits in the liver and tumors of other organs. Platelet additive solutions (PAS) are crystalloid nutrient media used for platelet storage in place of plasma. Platelet products are irradiated to prevent transfusion-associated graft-versus-host disease (TA-GvHD) and transfusion-transmitted infections. Transfused platelets have a short lifespan, requiring re-dosing within 3-4 days if given for prophylaxis. Irradiation of platelet products is done to prevent the proliferation of viable T lymphocytes, the cause of Transfusion Associated-Graft Versus Host Disease (TA-GVHD). PAS-positive lymphocytes can be found in certain diseases like Mycosis fungoides, Sézary syndrome, Ewing sarcoma, and erythroleukemia. These lymphocytes stain bright fuchsia.
Questions and Answers
1. What is PAS in blood?
PAS (Periodic Acid Schiff) Stain is an immunohistochemical method used on biopsies and as a diagnostic tool for blood specimens.
2. Are PAS platelets irradiated?
Psoralen-treated platelets do not require irradiation, so they do not have a Rad-Sure™ sticker on the bag.
3. What are psoralen-treated platelets?
Psoralen-treated platelets undergo a process where they are treated with a psoralen drug (amotosalen) followed by UV light to prevent DNA or RNA replication.
4. Can pathogen-reduced platelets be irradiated?
Platelets that have been pathogen-reduced by psoralen treatment and UVA do not need to be irradiated, as this method is considered equivalent to irradiation by the FDA.
5. What does PAS-positive mean?
PAS-positive substances include polysaccharides like glycogen, cellulose, and starch, which can be found in various organisms, including fungi, actinomycosis, and bacteria.
6. What does PAS-positive indicate?
The PAS staining procedure is commonly used to detect glycogen deposits in the liver when glycogen storage disease is suspected. Glycogen granules may also be visible in tumors of the bladder, kidney, ovary, pancreas, and lung.
7. What is a PAS solution for platelets?
Platelet additive solutions (PAS) are crystalloid nutrient media used for platelet storage in place of plasma. They reduce the amount of storage plasma in platelet components.
8. Why do platelets need to be irradiated?
Irradiation of platelet products is done to prevent transfusion-associated graft-versus-host disease (TA-GvHD) and transfusion-transmitted infections.
9. How long does platelet infusion last?
Transfused platelets have a short lifespan and may need to be re-dosed within 3-4 days if given for prophylaxis. Non-immune destruction or immune refractoriness can affect platelet increases.
10. Why do we irradiate platelet products?
Platelet products are irradiated to prevent the proliferation of viable T lymphocytes, which are the cause of Transfusion Associated-Graft Versus Host Disease (TA-GVHD).
11. What disease is PAS positive?
PAS-positive lymphocytes can be found in diseases like Mycosis fungoides, Sézary syndrome, Ewing sarcoma, and erythroleukemia. These lymphocytes stain bright fuchsia.
What is PAS in blood
PAS (Periodic Acid Schiff) Stain, Blood/Bone Marrow Hot
Periodic acid Schiff (PAS) staining is an immunohistochemical method employed on biopsies and also as a diagnostic device for blood specimens.
Are PAS platelets irradiated
As the psoralen-treated platelets do not require irradiation there will NOT be a Rad-Sure™ sticker on the bag. No irradiation will be performed on psoralen-treated platelets.
What is psoralen treated platelets
Psoralen treated platelets start with a standard apheresis platelet collection, which is then treated with a psoralen drug (amotosalen) followed by UV light. During this process, the psoralen intercalates into nucleic acids and becomes fixed, preventing DNA or RNA replication.
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Can pathogen reduced platelets be irradiated
Platelets that have been pathogen- reduced by psoralen treatment and UVA thus do not need to be irradiated. The FDA considers this method of pathogen reduction equivalent to irradiation.
What does PAS positive mean
The substances positive for PAS are: Polysaccharides: Glycogen, cellulose and starch. Many leucocytes contain glycogen, capsule of fungi (Candida albicans, Histoplasma capsualtum, Cryptococcus and Blastomycosis), actinomycosis and bacteria.
What does PAS positive indicate
The periodic acid-Schiff (PAS) staining procedure is most commonly used in the histology laboratory to detect glycogen deposits in the liver when glycogen storage disease is suspected. Glycogen granules may also be visible in tumors of the bladder, kidney, ovary, pancreas, and lung.
What is a pas solution for platelets
Platelet additive solutions (PAS) are crystalloid nutrient media used in place of plasma for platelet storage. They replace 60%–70% of plasma in platelet components, so the amount of storage plasma can be decreased.
Why do platelets need to be irradiated
Irradiation of platelet products is generally used to prevent transfusion-associated graft-versus-host disease (TA-GvHD) as well as transfusion-transmitted infections.
How long does platelet infusion last
Transfused platelets have a short life span and will need to be re-dosed within 3-4 days if given for prophylaxis. Suboptimal increases can be seen due to non-immune destruction or immune refractoriness.
Why do we irradiate platelets products
As described in the Technical Manual (20th Edition) and Circular of Information (October 2017), cellular blood components are irradiated prior to transfusion to prevent the proliferation of viable T lymphocytes which are the immediate cause of Transfusion Associated-Graft Versus Host Disease (TA-GVHD).
What disease is PAS positive
Aggregates of PAS-positive lymphocytes are present in epidermis in Mycosis fungoides and Sézary syndrome, called Pautrier microabscesses. Ewing sarcoma. Erythroleukemia, a leukemia of immature red blood cells. These cells stain a bright fuchsia.
What is PAS useful in the diagnosis of
From a diagnostic point of view, PAS is one of the most useful and used special stains in the pathology laboratory. It can help on the one hand to the differential diagnosis of tumors and on the other hand to detect fungal diseases.
Do you infuse platelets with normal saline
Platelets are transfused at the bedside through intravenous tubing with an in-line filter (screen filter of 170-260 micrometer pore size) to remove fibrin clots and large debris. The tubing can be primed with normal saline or blood product itself.
Who needs irradiated platelets
Immunocompromised patients such asInfants (particularly premature) up to 4, 6, or 12 months depending on institutional policy.Intrauterine transfusion* and/or neonatal exchange transfusion recipients.Congenital immunodeficiency disorders of cellular immunity (i.e., SCID, DiGeorge)*
Do all chemo patients need irradiated blood
People who've been treated with certain chemotherapy drugs, including fludarabine, cladribine, bendamustine and pentostatin, should have irradiated blood products for the rest of their lives.
What are the side effects of a platelet infusion
Fever, chills are the most common adverse events associated with platelet transfusion and are more common with red blood cell transfusion (leukopenia, which will decrease response Allergic reactions: pruritus, urticaria, anaphylaxis, and anaphylaxis are also more common with platelet transfusions than with red blood …
How do you feel after platelet infusion
One of the most common inflammatory reactions to platelet transfusion recipients is fever with or without rigours (FNHTR); most common symptoms are listed as follows: fever, chills and rigours.
Which patients need irradiated blood products
Immunocompromised patients such as
Fresh liquid plasma (never frozen) may have a small amount of viable lymphocytes and should be irradiated if the patient has indications for irradiated cellular blood products.
What does PAS mean in biopsy
Periodic acid-Schiff (PAS) is a staining method used to detect polysaccharides, manifested as glycogen, glycoproteins, glycolipids, and mucin in tissues.
What is PAS used for treatment of
PAS helps to demonstrate cerebrosides and gangliosides. It helps in diagnosis of Gaucher's disease, Krabbe's disease and lysosomal storage diseases etc.
What does PAS mean in healthcare
Personal assistance services (PAS) provider: The employee or independent contractor whose primary job functions include provision of personal assistance services.
Can you use regular IV tubing for platelets
Can I transfuse different products through regular blood tubing Yes, unless otherwise stated in the instructions for use on the blood tubing packaging you can transfuse red blood cells, platelets, plasma or cryo through the same filter set.
What are diluting fluids for platelets
Platelet diluting fluid consists of 1 % ammonium oxalate. This must be prepared using scrupulously clean glassware and glass distilled water. Not more than 500 ml should be prepared at a time.
Why would a patient need irradiated blood
It only stops white blood cells in the donated blood from causing you harm. Why do I need irradiated blood You are at risk for developing transfusion-associated graft-versus-host disease (TA-GvHD). Irradiated blood can stop you from getting this disease.
What happens when a patient receives non irradiated blood
The blood does not become 'radioactive' and will not harm you or anyone around you. What if blood is needed in an emergency Although irradiated blood is recommended for you, if you receive non-irradiated blood the risk of TA-GvHD is very small.